PowerStem ESPro 2, Serum-free Media for ES and stem cells, 500 ml Kit – P04-77020K

PowerStem ESPro2 is a fully defined, serum-free medium for cultivation and expansion of embryonic stem cells of mice (mES cells). PowerStem ESPro2 is especially designed to proliferate and expand mES cells without differentiation. To differentiate the proliferated mES cells into different cell types the relevant protocols and differentiation factors can be used.

PowerStem ESPro2 basal medium, PowerStem ESPro2 growth supplement and PowerStem ESPro2 LIF supplement are guaranteed stable for 12 months when properly stored. PowerStem ESPro2 complete medium (basal + supplements) is stable for 1 month when stored in the dark at 2-8° C. We do not recommend using the complete medium beyond 1 month.

PowerStem ESPro2 contains purified proteins, lipids, salts, amino acids, trace elements, attachment factors, hormones and growth factors in an optimized formulation. PowerStem ESPro2 is fully defined and contains no peptones or hydrolysates.

Please note: Supplemented PowerStem ESPro2 contains LIF in a concentration of 10μg/l. If higher levels of LIF are required, please add additional LIF to the medium.

PowerStem ESPro2 is especially designed for the serum-free cultivation of murine embryonic stem cells (mES cells), while maintaining the undifferentiated state. PowerStem ESPro2 is suitable for the serum-free generation of knockout-mice from genetically modified mES cells. PowerStem ESPro2 has also been proven to support the serum-free cultivation and expansion of tumor progenitor cells.

PowerStem ESPro2 allows the cultivation and expansion of mouse embryonic stem cells (mES cells) under serum-free conditions. It is fully defined in its composition and thus enables constant and comparable experimental conditions resulting in highly reproducible data. The mES cell culture can be established without the usual feeder layer (primary fibroblasts). Cells show a high proliferation rate and largely retain an undifferentiated state. By adding specific differentiation factors, mES cells can differentiate in vitro to the desired cell types (e.g. nerve cells, muscle cells, endothelial cells, etc.).

Please note: For differentiation studies LIF supplement must be omitted.