PowerStem iPS2, 500 ml Kit – P04-77140K

PowerStem iPS2 is a specialized serum-free, fully defined medium for cultivation and expansion of human induced pluripotent stem cells (iPS cells). Human induced pluripotent stem cells have the capacity to differentiate into all of the somatic cell types and therefore hold great promise for regenerative medicine. Even after long-term culture (> 50 passages) iPS cells retain a normal karyotype and a stable proliferating rate.

PowerStem iPS2 basal medium and PowerStem iPS2 growth supplement are guaranteed stable for 12 months when properly stored. PowerStem iPS2 complete medium (basal + supplement) is stable for 1 month when stored in the dark at 2-8° C. We do not recommend using the complete supplemented medium beyond 1 month.

PowerStem iPS2 contains purified and recombinant proteins, lipids, salts, amino acids, trace elements, attachment factors, hormones and growth factors in an optimized formulation. PowerStem iPS2 is fully defined and contains no animal-derived components.

Please note: PowerStem iPS2 growth supplement contains a high amount of growth factors. To avoid freeze-thaw cycles, the growth supplement is provided as 5 x 1ml aliqouts.

Serum-free cultivation of human induced pluripotent stem cells (iPS cells), while maintaining an undifferentiated state.

PowerStem iPS2 allows the cultivation and expansion of iPS cells under serum-free conditions. It is fully defined in its composition thus enabling constant and comparable experimental conditions resulting in highly reproducible data. The iPS cells can be cultivated without the usual feeder layer of primary fibroblasts, they show a high proliferation rate and largely retain their undifferentiated state. By adding specific differentiation factors, iPS cells can be differentiated in vitro to the desired cell types (e.g. nerve cells, muscle cells, endothelial cells, etc.).

Please note: iPS cells grown in culture are exposed to a constant selection pressure of proliferation vs. differentiation.