PowerStem HE2, serum-free medium for human embryonic stem cells, 500 ml Kit – P04-77120K

PowerStem HE2 is a specialized serum-free, chemically defined medium for cultivation and expansion of human embryonic stem cells (hES cells). Pluripotent human embryonic stem cells have the capacity to differentiate into all of the somatic cell types and therefore hold great promise for regenerative medicine. Even after long-term culture, cells maintained on Vitronectin or Matrigel^TM retain a normal karyotype and a stable proliferating rate.

PowerStem HE2 basal medium and PowerStem HE2 growth supplement are guaranteed stable for 12 months when properly stored. PowerStem HE2 complete medium (basal + supplement) is stable for 1 month when stored in the dark at 2-8° C. We do not recommend using the complete medium beyond 1 month.

PowerStem HE2 contains purified and recombinant proteins, lipids, salts, amino acids, trace elements, attachment factors, hormones and growth factors in an optimized formulation. PowerStem HE2 is chemically defined and contains no animal-derived components.

Please note: PowerStem HE2 growth supplement contains a high amount of growth factors. To avoid freeze-thaw cycles, the growth supplement is provided as 5x 1ml aliquots.

Serum-free cultivation of human embryonic stem cells (hES cells), while maintaining an undifferentiated state.

PowerStem HE2 allows the cultivation and expansion of hES cells under serum-free conditions. It is fully defined in its composition thus enables constant and comparable experimental conditions resulting in highly reproducible data. The hES cells can be cultivated without primary fibroblast feeder layers. They show a high proliferation rate and largely retain their undifferentiated state. By adding specific differentiation factors, hES cells can be differentiated in vitro to the desired cell types (e.g. neurons, muscle cells, endothelial cells, etc.).

Please note: hES grown in culture are exposed to a constant selection pressure of proliferation vs. differentiation.