Pancell T is a chemically defined medium for serum-free cultivation and proliferation of lymphocytes from full blood. The medium can be used for the enrichment and expansion of T-cells in vitro.

It allows

  • Enrichment of T-cells from PBMC / full blood, after stimulation (e.g., with PHA-L or anti CD3)
  • Stable performance > 4 passages
  • Long-term cultivation of immortalized T-cells.
  • CD3+ > 80%
Composition
Pancell T consists of a balanced mixture of salts, amino acids, vitamins, trace elements, hormones for optimized growth. We also provide kit including PHA-L as supplement for the stimulation of lymphocytes (P05-01010M) and a cytokines supplement P04-413S.
Storage conditions and stability
Storage: 2-8°C in the dark
Stability: 10 months