Laminin from mouse, 1 mg – P06-20501

This highly purified preparation of mouse Laminin I increases cell adhesion, migration, growth, and differentiation. It is composed of 111 chains with a total MW of 800 kD and is used for the coating of culture dishes.

Source: Murine Engelbreth-Holm-Swarm (EHS) tumor
Storage Buffer: Dulbecco‘s Modified Eagle‘s Medium with 10 μg/ml gentamycin sulfate
Storage: Store at -20° C or at -80° C in a manual defrost freezer
Purity: Purity > 90 % by SDS-PAGE

Functional assays
Supports the formation of neuronal filaments of NG108-15 cells in a neurite outgrowth assaySterility testing

• No bacterial or fungal growth detected after incubation at 37° C for 14 days following USP XXIV, Chapter 71 sterility testing
• No mycoplasma contamination detected by PCR
• Endotoxin concentrations < 20 EU/ml by LAL assay

The recommended working concentration is 0.05 – 10 µg/cm² of growth surface (0.05 – 10 µg/ml) depending on cell type.

a. Thaw stock solution on ice for several hours. Place plates on ice and pre-chill pipette tips.
Distribute the solution to completely cover the bottom of the wells.

b. The following table gives suggested volume required per well:

c) Incubate the plates at 37° C for 1 hour. In the laminar flow hood, remove excess liquid from the wells of the tissue culture plate.

Rinse the wells once with tissue culture medium and then add your cells.