Cryopan I, Serum-free Freezing Medium, w/o: Human and Animal Components, 500 ml – P07-92500

SKU: P07-92500 Category:

Description

Cryopan I is a serum-free freezing medium for the cryoconservation of cells in a nitrogen storage tank or an ultra-low temperature freezer (< -150° C). It contains DMSO.

 

Freezing procedure
  • Refrigerate freezing medium, culture medium and freezing tubes!
  • Trypsinize cells, transfer the cells into the culture medium and centrifuge. Discard the supernatant and resuspend in culture medium
  • Cell count should be adjusted to 1 – 5 x 106/ml. The cells should be carefully resuspended to avoid clustering
  • Spin down and resuspend the cells in an appropriate volume of cool freezing medium by pipetting only once or twice. Distribute 1 ml cell suspension per freezing tube
  • To achieve a defined freezing rate of about 1° C per minute, manual freezing devices or computer controlled freezing may be used

 

Ideal freezing rate: decrease of 1° C per minute

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